Reverse transcription method

Details Reverse transcription method Reverse transcription method

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2291855

A method of improving the synthesis of full-length cDNA transcripts by Mn++- dependent reverse transcriptases, preferably DNA-dependent DNA polymerases, is disclosed. The improvement consists in the polymerisation in the presence of betaine.

Ce procédé consiste en une amélioration de la synthèse de transcription d'ADNc en pleine longueur par transcriptases inverses liées au Mn<++>, de préférence des polymérases d'ADN liées à l'ADN. L'amélioration tient à la polymérisation en présence de bétaïne.

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