Reverse two-hybrid systems

Details Reverse two-hybrid systems Reverse two-hybrid systems

C - Chemistry, Metallurgy

12

N

C12N 15/81 (2006.01) C12N 1/19 (2006.01) C12N 15/10 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2217545

Disclosed are methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are useful in practicing the methods of the invention.

L'invention concerne des procédés pour identifier des interactions moléculaires (par exemple protéine/protéine, protéine/ADN, protéine/ARN ou ARN/ARN). Tous ces procédés de l'invention font appel à une contre-sélection et à au moins deux molécules hybrides. Les molécules en interaction reconstituent un facteur de transcription et l'expression directe d'un gène marqueur, cette expression étant ensuite évaluée. L'invention concerne également des produits de recombinaison génétique servant à la mise en oeuvre des procédés de l'invention.

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