Reversible cross-linked hydrogels

C - Chemistry – Metallurgy – 08 – B

Patent

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Details

C08B 37/02 (2006.01) A61F 2/02 (2006.01) A61L 27/20 (2006.01) C08B 37/04 (2006.01) C08J 3/24 (2006.01) C08L 5/04 (2006.01)

Patent

CA 2392419

A hydrogel composition and methods of preparing and using the same are disclosed. The hydrogel composition comprises an oxidized polysaccharide, and a cross-linker having at least two functional cross-linking groups. The cross- linker reversibly cross-links the polysaccharide and is provided in an amount to provide dangling cross-linkers. The method of preparing a hydrogel comprises the steps of providing an oxidized polysaccharide, and providing a cross-linker having at least two functional cross-linking groups. The cross- linker is mixed with the polysaccharide at a concentration of the cross-linker sufficient to form a hydrogel wherein the cross-linker reversibly cross-links the polysaccharide and has dangling cross-linkers. The hydrogels are useful, for example, for tissue engineering, cell transplantation and drug delivery applications.

L'invention concerne une composition hydrogel et ses méthodes de préparation et d'utilisation. La composition hydrogel comprend un polysaccharide oxydé et un agent de réticulation présentant au moins deux groupes de réticulation fonctionnels. L'agent de réticulation se lie de manière réversible au polysaccharide et est présent en quantité suffisante afin de permettre la production d'agents effectuant des réticulations partielles. La méthode de préparation d'un hydrogel comprend les étapes consistant à fournir un polysaccharide oxydé et un agent de réticulation présentant au moins deux groupes de réticulation fonctionnels. L'agent de réticulation est mélangé avec le polysaccharide en une concentration d'agent de réticulation suffisante afin de créer un hydrogel, l'agent de réticulation se liant de façon réversible au polysaccharide et possédant des réticulations partielles. Ces hydrogels conviennent, par exemple, pour l'ingénierie tissulaire, la transplantation de cellules et des applications consistant à administrer des médicaments.

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