Reversible flow chromatographic binding assay

Details Reversible flow chromatographic binding assay Reversible flow chromatographic binding assay

G - Physics

01

N

G01N 33/569 (2006.01) B01L 3/00 (2006.01) G01N 33/558 (2006.01) G01N 30/02 (2006.01) G01N 30/40 (2006.01) G01N 30/90 (2006.01) G01N 37/00 (2006.01)

Patent

CA 2113351

Disclosed are devices and methods for performing an assay which determines the presence or quantity of an analyte in a fluid sample by detecting binding of the analyze to at least one immobilized analyze capture reagent and washing unbound mate- rial from the immobilized analyze capture reagent. The devices and methods involve an elongated solid phase flow matrix, includ- ing capillary channels capable of driving capillary fluid movement, and further including (i) a first region adapted for receipt of the fluid sample, (ii) a second region at which the analyze capture reagent is immobilized, (iii) a third region for application of a wash reagent capable of removing unbound substances from the second region; and (iv) an absorbent reservoir of high volume capacity. The second region is positioned intermediate to the first region and the third region and intermediate to the absorbent reservoir and the third region. The device also includes means to detect analyze bound at the second region. The flow matrix and the regions thereof are sized and positioned to cause the fluid sample to flow initially along the elongated flow matrix in one di- rection toward and through the second region, and subsequently, the liquid reagent to flow along the elongated flow matrix in a second direction opposite the first direction, through the second region, and into the absorbent reservoir, drawing unbound sub- stances with it.

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