Reversible immortalization of cells

Details Reversible immortalization of cells Reversible immortalization of cells

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) A61K 35/12 (2006.01) A61K 48/00 (2006.01) A61P 43/00 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 9/12 (2006.01) C12N 15/12 (2006.01) C12N 15/37 (2006.01) C12N 15/38 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2406171

A gene complex for reversible immortalization of cells contains an immortalizing gene domain including at least one resistance gene, an immortalization gene and preferably a suicide gene, in addition to sequences flanking said gene domain and acting as a recognition point for homologous, intermolecular recombination, as well as at least one promoter (fig. 1) which prefixes the gene domain. A gene complex for immunomodulation of cells contains a first immunomodulated gene domain whose expression inhibits the function of MHC I molecules, a second immunomodulating gene domain whose expression results in an inactivation of natural killer cells, and a resistance gene. Organ-related cells are provided according to a method for obtaining cells; said cells are immortalized by transferring the first gene complex and are immunomodulated by transferring the second gene complex. The immortalization is reversed after expansion of the immortalized cells has occurred.

Un complexe génique servant l'immortalisation réversible de cellules contient un domaine génique d'immortalisation qui présente au moins un gène de résistance, un gène d'immortalisation et de préférence un gène suicide, ainsi que deux séquences flanquant le domaine génique et faisant office de site de reconnaissance pour des recombinaisons intramoléculaires homologues, et au moins un promoteur placé avant le domaine génique (cf. fig). Un complexe génique pour l'immunomodulation de cellules contient un premier domaine génique d'immunomodulation dont l'expression inhibe la fonction de molécules MHC-I, un deuxième domaine génique d'immunomodulation dont l'expression provoque une inactivation de cellules NK, et un gène de résistance. Un procédé d'obtention de cellules permet d'obtenir des cellules spécifiques à un organe, qui sont immortalisées par transfert du premier complexe génique et immunomodulées par transfert du deuxième complexe génique. L'immortalisation est supprimée, après expansion des cellules immortalisées,.

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