Reversible mhc multimer staining for functional purification...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/071 (2010.01) C12N 5/0783 (2010.01) C07K 14/74 (2006.01) C07K 19/00 (2006.01) C12Q 1/04 (2006.01) G01N 33/50 (2006.01) G01N 33/567 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2467434

The present invention relates to a new method for reversible staining and functional isolation or characterization of cells, e.g. antigen-specific T cells. With this technique, the original functional status of cells can be substantially maintained after their identification and purification. Thus, this new method is of broad benefit for basic research and clinical applications.

L'invention concerne un nouveau procédé de coloration réversible et d'isolation ou de caractérisation fonctionnelle de cellules, p. ex. des lymphocytes T spécifiques de l'antigène. Grâce à cette technique, le statut fonctionnel original de cellules peut être sensiblement maintenu après leur identification et purification. Ce nouveau procédé représente ainsi un grand avantage dans la recherche fondamentale et les applications cliniques.

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