Reversible, parallel and multitask cloning method and kit

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/10 (2006.01) C12N 15/65 (2006.01) C12N 15/66 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2496514

The present invention is related to a reversible, parallel and/or multitask cloning method and kit, which improve the cloning of (preferably multiple) genetic element(s) in a nucleic acid construct such as vector or in chromosome of a cell and the rapid and efficient selection of said construct with a correct integration of said genetic element(s) either in vitro or in vivo.

La présente invention concerne un procédé et un nécessaire de clonage réversible, parallèle et multitâche, qui améliore, d'une part le clonage d'au moins un élément génétique, mais de préférence plusieurs éléments génétiques dans une construction d'acide nucléique telle qu'un vecteur, ou dans un chromosome d'une cellule, et d'autre part la sélection rapide et efficace de ladite construction, avec une intégration correcte de ces éléments génétiques, in vitro ou in vivo.

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