Rhamnose binding protein

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/705 (2006.01) A61K 38/17 (2006.01) A61K 39/395 (2006.01) A61P 35/00 (2006.01) C07K 16/30 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2475066

An isolated RBP with at least one of the following characteristics: a) a molecular weight of approximately 65-70 kDa and more preferably 66-69kDa; b) a pI of greater than 10 or less than 3; c) a dissociation constant of approximately 1.5 x 10-6 when bound to the rhamnose moiety of solamargine; d) adapted to bind to a rhamnose affinity column prepared according to example 1 and under the conditions set out therein ; e) adapted to be eluted from the column in example 1 with a 100mM rhamnose solution; f) insoluble in aqueous solution; and g) soluble in highly denaturing buffers containing greater that approximately 2% surfactant.

L'invention concerne une protéine fixant le rhamnose RBP présentant au moins une des caractéristiques suivantes: a) une masse moléculaire d'approximativement 65-70 kDa et idéalement de 66-69kDa; b) un pl supérieur à 10 ou inférieur à 3; c) une constante de dissociation d'approximativement 1,5 x 10?-6¿ lors d'une fixation à la fraction rhamnose de solamargine; d) une aptitude à se fixer à une colonne d'affinité de rhamnose préparée selon l'exemple 1 et dans les conditions y figurant; e) une aptitude à être éluée à partir de la colonne dans l'exemple 1 avec une solution à 100 mM de rhamnose; f) une insolubilité en solution aqueuse; et g) une solubilité dans des tampons hautement dénaturant contenant plus d'approximativement 2 % de tensioactif.

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