Rho antibody and tag to purify cell surface proteins

Details Rho antibody and tag to purify cell surface proteins Rho antibody and tag to purify cell surface proteins

C - Chemistry, Metallurgy

07

K

C07K 1/00 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2539664

A process for the expression and purification of membrane associated proteins is provided. A process for the expression and purification of membrane proteins such as G-protein coupled receptors is provided. A hybrid polypeptide is constructed from a protein of interest and a peptide tag from the amino- terminus extracellular domain of rhodopsin. A method for purifying the hybrid protein using the rhodopsin tag is provided. Methods of detecting the expressed hybrid are also provided.

L'invention concerne un procédé permettant d'exprimer et de purifier des protéines associées à des membranes. Elle concerne également un procédé permettant d'exprimer et de purifier des protéines membranaires telles que des récepteurs couplés à la protéine G. Un polypeptide hybride est construit à partir d'une protéine d'intérêt et une étiquette peptidique à partir du domaine extracellulaire de la terminaison amino de la rhodopsine. L'invention concerne en outre un procédé permettant de purifier la protéine hybride au moyen de l'étiquette de la rhodopsine, ainsi que des procédés de détection de l'hybride exprimé.

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