C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/31 (2006.01) A61K 39/02 (2006.01) C07H 21/04 (2006.01) C07K 14/195 (2006.01) C07K 14/34 (2006.01) C07K 14/36 (2006.01) C07K 16/12 (2006.01) A61K 39/00 (2006.01)
Patent
CA 2125426
This invention relates to Rhodococcus equi bacteria immunogenic proteins on the bacterial cell surfaces, genetic information as it relates to the proteins and diagnostics and vaccine components based on the use of such proteins. The DNA sequence encoding the virulent protein is described along with the corresponding amino acid sequence for the protein. The protein, as expressed by R. equi, includes a signal peptide portion which is cleaved off in the process of the protein being excreted through the cell wall. Also in that process, the protein is lipid modified to provide proteins of SDS-PAGE gel estimated molecular weights of 17 through 22 kDa. A plasmid construct is provided suitable for expression in E. coli host for providing the protein. Antibodies have been raised to the protein which are useful in diagnostic and passive vaccines. Active vaccines are provided based on the use of the protein. The protein is hydrophobic in nature when lipid modified. Hence, a process for recovery of the hydrophobic protein is provided which involves the use of a detergent.
Prescott John F.
Tan Cuiwen
Bereskin & Parr
University Of Guelph
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