Riboflavin biosynthesis genes from plants and uses thereof

Details Riboflavin biosynthesis genes from plants and uses thereof Riboflavin biosynthesis genes from plants and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 9/14 (2006.01) C12N 15/54 (2006.01) C12N 15/55 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2318522

The present invention provides plant riboflavin biosynthesis genes, including a gene that encodes the .beta. subunit of the plant riboflavin synthase enzyme complex (lumazine synthase) and a gene that encodes the bifunctional enzyme GTP cyclohydrolase II/DHBP synthase. Also disclosed are the recombinant production of these plant riboflavin biosynthesis enzymes in heterologous hosts, screening chemicals for herbicidal activity using these recombinantly produced enzymes, and the use of thereby identified herbicidal chemicals to suppress the growth of undesired vegetation. Furthermore, the present invention provides methods for the development of herbicide tolerance in plants, plant tissues, plant seeds and plant cells using the riboflavin biosynthesis genes of the invention.

L'invention concerne des gènes de biosynthèse de la riboflavine extraits de plantes, qui comprennent un gène encodant la sous-unité bêta du complexe enzymatique de la riboflavine synthase de la plante (lumazine synthase), et un gène encodant l'enzyme bifonctionnelle GTP cyclohydrolase II/DHBP synthase. L'invention concerne également la production recombinante desdites enzymes biosynthétiques de la riboflavine extraites de plantes dans des hôtes hétérologues, le criblage de produits chimiques au moyen desdites enzymes obtenues par recombinaison pour déceler une activité herbicide, et l'utilisation de produits chimiques herbicides ainsi identifiés pour freiner la croissance d'une végétation non désirée. L'invention concerne en outre des procédés de mise au point d'une tolérance aux herbicides dans des plantes, tissus de plantes, semences de plantes et cellules de plantes, au moyen desdits gènes de biosynthèse de la riboflavine.

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