Ribonucleotide tag nucleic acid detection

Details Ribonucleotide tag nucleic acid detection Ribonucleotide tag nucleic acid detection

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12M 1/34 (2006.01) C12M 1/38 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2663302

The present application provides polynucleotides comprising 5'-tails with sequence segments useful for the detection of target nucleic acid sequences, and methods for their use in detecting target nucleic acids. The polynucleotides are used to amplify a subsequence of a target nucleic acid in the presence of one or more ribonucleotides. The ribonucleotides are incorporated into amplification products at regular intervals complementary to the 5'-tail sequence segments. Cleavage of amplification products at the bond immediately 3' to incorporated ribonucleotides produces detectably distinct fragments indicative of the presence or absence of a target nucleic acid.

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