Ribosome display or mrna display method with selection for...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/10 (2006.01)

Patent

CA 2593151

A method of providing a subject polypeptide variant with improved stability compared with a parent subject polypeptide, employing translation and selection using an RNA expression system, wherein two or more stability selection pressures are applied simultaneously during translation, two or more stability selection pressures are applied simultaneously during selecting, or at least one stability selection pressure is applied during translation and continues to be applied during selecting, and at least one further stability selection pressure is applied during selecting.

Ci-décrit est un procédé d'obtention d'un variant polypeptidique de l'invention ayant une stabilité améliorée par comparaison avec un polypeptide de l'invention parent, ledit procédé employant la traduction et la sélection à l'aide d'un système d'expression d'ARN, et étant caractérisé en ce que deux pressions de sélection de stabilité ou plus sont appliquées simultanément pendant la traduction, en ce que deux pressions de sélection de stabilité ou plus sont appliquées simultanément pendant la sélection, ou en ce qu'au moins une pression de sélection de stabilité est appliquée pendant la traduction et continue d'être appliquée pendant la sélection, et au moins une pression de sélection de stabilité supplémentaire est appliquée pendant la sélection.

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