Ribozymes with broad trna aminoacylation activity

C - Chemistry – Metallurgy – 07 – H

Patent

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Details

C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/00 (2006.01) C12N 15/11 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2476425

The present invention provides catalytic RNA molecules having cis or trans aminoacylation activity. The catalytic RNA molecules having cis aminoacylation activity comprise a catalytic domain and an aminoacylation domain. The catalytic RNA molecules having trans aminoacylation activity only have the catalytic domain. A method is provided for constructing and screening of these molecules. These molecules are suitable for aminoacylating with specific amino acids.

La présente invention concerne des molécules d'ARN catalytique présentant une activité de cis- ou de trans-aminoacylation. Les molécules d'ARN catalytique à activité de cis-aminoacylation comprennent un domaine catalytique et un domaine d'aminoacylation. Les molécules d'ARN catalytique à activité de trans-aminoacylation ne comprennent que le domaine catalytique. L'invention concerne également un procédé permettant la construction et le criblage de ces molécules. Ces molécules conviennent pour l'aminoacylation avec des acides aminés spécifiques.

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