Rna-dependent dna polymerase from geobacillus...

Details Rna-dependent dna polymerase from geobacillus... Rna-dependent dna polymerase from geobacillus...

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C12N 1/21 (2006.01) C12N 9/12 (2006.01) C12N 15/10 (2006.01) C12N 15/31 (2006.01) C12N 15/63 (2006.01) C12P 19/34 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2553734

The invention provides an isolated polynucleotide sequence from the genome of Bacillus stearothermophilus (Geobacillus stearothermophilus) and an amino acid sequence encoded by he polynucleotide sequence, the corresponding amino acid sequence comprising a novel enzyme, Tirt (thermostable intron reverse transcriptase), having reverse transcriptase activity and retaining that activity at temperatures of up to about 75~C.

Séquence de polynucléotides isolée tirée du génome de Bacillus stearothermophilus (Geobacillus stearothermophilus) et séquence d'acides aminés codée par ladite séquence de polynucléotides, la séquence d'acides aminés correspondante comportant une nouvelle enzyme, Tirt (thermostable intron reverse transcriptase / transcriptase inverse thermostable à introns), qui possède une activité de transcriptase inverse et conserve cette activité à des températures allant jusqu'à environ 75 ·C.

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