Rna detection enzymes

Details Rna detection enzymes Rna detection enzymes

C - Chemistry, Metallurgy

12

N

C12N 9/22 (2006.01) C12Q 1/44 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2477698

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5' nuclease activity of a variety of enzymes is used to cleave a target- dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

L'invention concerne de nouveaux agents de clivage et de nouvelles polymérases destinés au clivage et à la modification de l'acide nucléique. Ces agents et ces polymérases peuvent se révéler utiles, par exemple, pour la détection et la caractérisation de séquences d'acide nucléique et de variations de séquences d'acide nucléique. Dans certains modes de réalisation, l'activité 5' nucléase d'une variété d'enzymes permet de cliver une structure de clivage dépendant de la cible, ce qui indique la présence de séquences d'acide nucléique spécifiques ou de variations spécifiques de ces séquences.

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