Rna polyphosphatase compositions, kits, and uses thereof

Details Rna polyphosphatase compositions, kits, and uses thereof Rna polyphosphatase compositions, kits, and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 9/14 (2006.01) C12Q 1/42 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2723235

The present invention relates to the discovery of RNA 5' polyphosphatase enzymes not previously described in the art, methods for discovery of said enzymes, compositions of said enzymes, methods for making said enzymes, and various meth-ods and kits for using said enzymes for biomedical research, for human and non- human diagnostics, for production of therapeutic products, and for other applications. In particular, some embodiments provide compositions, kits and methods for employing RNA polyphosphatases for isolation, purification, production, and assay of capped RNA using a biological sample or a sample from an in vitro capping reaction wherein the sample also contains RNA that is not capped. Other embodiments provide composi-tions, kits and methods wherein RNA polyphosphatases comprise signal-amplifying enzymes for analyte-specific assays.

L'invention concerne la découverte d'enzymes d'ARN 5' polyphosphatase non décrits précédemment dans la technique, des procédés relatifs à la découverte desdits enzymes, leurs compositions, et divers procédés et trousses d'utilisation de ces enzymes dans la recherche biomédicale, les diagnostics humains et non humains, la production de produits thérapeutiques, et d'autres applications. Plus précisément, certains modes de réalisation concernent des compositions, des trousses et des procédés permettant d'employer des polyphosphatases pour l'isolement, la purification, la production, et l'analyse d'ARN coiffé à l'aide d'un échantillon biologique ou un échantillon prélevé dans une réaction de coiffage in vitro dans laquelle l'échantillon contient également un ARN non coiffé. D'autres modes de réalisation concernent en outre des compositions, des trousses et des procédés où les ARN polyphosphatases comprennent des enzymes amplificateurs de signaux pour dosages spécifiques d'analytes.

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