Rnai for control of insects and arachnids

Details Rnai for control of insects and arachnids Rnai for control of insects and arachnids

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) A01N 63/00 (2006.01) A01P 7/04 (2006.01) A61K 31/713 (2006.01) C07K 14/435 (2006.01) C12N 1/21 (2006.01)

Patent

CA 2610644

The present invention describes a new non-compound based approach for insect and/or arachnid control. The present inventors have identified for the first time novel targets for RNAi, which can effectively control insect and/or arachnid pest populations. Accordingly, the invention provides both nucleotide and amino acid sequences for the novel targets. Also provided are RNA constructs including double stranded RNA regions for mediating RNAi in insects, DNA constructs, expression vectors, host cells and compositions for controlling insects and/or arachnids using RNAi. Finally, the invention also provides for the use of the constructs, vectors, host cells and compositions in control of insects and/or arachnids populations and suitable kits for use in an RNAi based method of controlling insect and/or arachnid pests.

Une nouvelle approche non centrée sur les composés pour la lutte contre les insectes et/ou les arachnides. De nouvelles cibles pour ARNi ont été identifiées pour la première fois, elles s'avèrent utiles dans la lutte efficace contre les populations d'insectes et/ou d'arachnides. Dans ce sens, on prévoit à la fois des séquences nucléotidiques et acides aminés pour les nouvelles cibles et des gènes hybrides d'ARN comprenant des régions d'ARN à double brin pour médier l'ARNi chez les insectes, les gènes hybrides d'ADN, les cellules hôtes, les vecteurs d'expression et les compositions de lutte contre les insectes et/ou les arachnides utilisant l'ARNi. Finalement, on prévoit l'utilisation des gènes hybrides, des vecteurs, des cellules hôtes et des compositions dans la lutte contre les populations d'insectes et/ou d'arachnides et des kits adéquats à utiliser dans ce procédé.

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