C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/22 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2722541
The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3' end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.
L'invention concerne de nouveaux composés oligonucléotidiques pour utilisation dans divers dosages biologiques, tels que des réactions d'amplification d'acide nucléique, de ligation et de séquençage. Les nouveaux oligonucléotides comprennent un domaine d'acide ribonucléique et un groupe de blocage à l'extrémité 3' de l'oligonucléotide ou à proximité de celle-ci. Ces composés offrent un niveau accru de spécificité précédemment non constaté. Les procédés pour effectuer une amplification d'acide nucléique, une ligation et un séquençage sont également fournis. De plus, des kits contenant les oligonucléotides sont également décrits ici.
Behlke Mark Aaron
Dobosy Joseph
Rose Scott
Walder Joseph Alan
Gowling Lafleur Henderson Llp
Integrated Dna Technologies Inc.
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