Room temperature storage of organs

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/00 (2006.01) C12N 5/10 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2527822

The present invention relates generally to compositions and method for freezing and/or drying organs for storage prior to use. In particular, the present invention relates to the genetic modification of cells used to form organs so that organs formed from the genetically modified cells can be dried. According to the invention, mammalian cells may be modified with genes encoding plant late embryogenesis protein HVAI, trehalose transport protein, or a trehalose synthesis pathway. The invention also provides methods of treating patients with organs that have been preserved by freezing and/or drying.

L'invention concerne de manière générale des compositions et un procédé de congélation et/ou de séchage d'organes en vue de leur stockage avant utilisation. Elle concerne en particulier la modification génétique de cellules utilisées pour former des organes, afin que les organes formés à partir des cellules génétiquement modifiées puissent être séchés. Selon l'invention, des cellules de mammifère peuvent être modifiées à l'aide de gènes codant pour la protéine HVA1 d'embryogenèse végétale tardive, la protéine de transport du tréhalose ou une voie de synthèse du tréhalose. L'invention concerne aussi des méthodes de traitement de patients au moyen d'organes ayant été conservés par congélation et/ou séchage.

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