Rotary thermocycling apparatus

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/40 (2006.01) B01L 7/00 (2006.01) C12M 1/38 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2255850

A rotary thermocycling apparatus, especially for biochemical reactions. The apparatus comprises a plurality of and especially at least four stations for receiving biochemical samples in a flat-bottomed container. Each station has a flat heated plate on which the container is placed and means to independently control the heated plate at a pre-determined temperature. The apparatus has means to move each flat-bottomed container from one station to another station in a pre-determined sequence. At least two of the stations have a heating unit adapted to be lowered over a container located on the station, and at least one station has a spray unit adapted to spray liquid reagents) into a container located at that one station. A method is also described. The apparatus is used in, for example, polymerase chain reactions.

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