Rotavirus antigens, vaccines and diagnostic drugs for...

C - Chemistry – Metallurgy – 12 – N

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C12N 7/00 (2006.01) A61K 39/15 (2006.01) C12N 5/06 (2006.01) C12N 7/04 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2240843

A process for producing rotavirus antigens characterized by cloning, from cultured cells, those allowing an ample growth of rotavirus which can be hardly cultured on a large scale, subculturing and conditioning the rotavirus in the cell clone strain thus obtained to thereby give a cell clone- conditioned rotavirus strain, culturing as a seed virus this strain or a reassortant constructed by using the same as the parent strain, and then isolating and purifying a rotavirus antigen from the culture; and vaccines and diagnostic drugs for infection with rotavirus produced by using these antigens. These antigens, vaccines and diagnostic drugs largely contribute to fundamental studies and clinical application relating to infection with rotavirus.

Procédé servant à préparer des antigènes de rotavirus et consistant à cloner, à partir de cultures de cellules, les cellules qui assurent une croissance importante de rotavirus difficile à cultiver sur une grande échelle, à effectuer une deuxième culture et un traitement du rotavirus dans la souche obtenue du clone de cellule, ce qui permet d'obtenir une souche de rotavirus traitée à partir du clone cellulaire, à cultiver cette souche en tant que semence de virus ou une souche obtenue par génie génétique en utilisant la souche mère, puis à isoler et à purifier un antigène de rotavirus à partir de cette culture; vaccins et médicaments permettant de diagnostiquer l'infection par rotavirus préparés au moyen de ces antigènes. Ces antigènes, ces vaccins et ces médicaments de diagnostic contribuent considérablement aux études fondamentales et à l'application clinique concernant les infections par rotavirus.

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