C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 19/04 (2006.01) A23L 1/03 (2006.01) A61K 31/715 (2006.01) C12M 1/40 (2006.01) C12N 9/10 (2006.01) C12N 11/00 (2006.01) C12P 19/18 (2006.01) C12P 19/26 (2006.01)
Patent
CA 2062715
2062715 9116449 PCTABS00008 A method for preparing saccharide compositions is disclosed. The method is reiterative and comprises the following three steps: (i) a glycosyltransferase capable of transfering a preselected saccharide unit to an acceptor moiety is isolated by contacting the acceptor moiety with a mixture suspected of containing the glycosyltransferase under conditions effective to bind the acceptor moiety and the glycosyltransferase and thereby isolate the glycosyltransferase. The acceptor moiety is a protein, a glycoprotein, a lipid, a glycolipid, or a carbohydrate. (ii) The isolated glycosyltranferase is then used to catalyze the bond between the acceptor moiety and the preselected saccharide unit. (iii) Steps (i) and (ii) are repeated a plurality of times with the intermediate product obtained in the first iteration of the method being used as the acceptor moiety of the second iteration.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
The Trustees Of The University Of Pennsylvania
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