Saccharomyces cerevisiae yeast strain with improved phytase...

Details Saccharomyces cerevisiae yeast strain with improved phytase... Saccharomyces cerevisiae yeast strain with improved phytase...

C - Chemistry, Metallurgy

12

N

C12N 9/16 (2006.01) A21D 8/04 (2006.01) C12N 1/19 (2006.01)

Patent

CA 2517582

The present invention relates to a process for preparing a Saccharomyces cerevisiae yeast strain having improved phytase activity, the strain being modified by inserting one or more of the genes PHO5, PHP10, PHO11, PHO2, PHO4, PHO23, and/or DIA3 to overexpress said genes, and/or by deleting one or more of the regulatory, repressor genes PHO80 and/or PHO85, whereby the insertion is either chromosomal or by transforming the strain using a plasmid containing said gene/s.

L'invention concerne un procédé pour préparer une souche de levure saccharomyces cerevisiae à activité de la phytase améliorée. Cette souche est modifiée par insertion d'un ou de plusieurs gènes PHO5, PHP10, PHO11, PHO2, PHO4, PHO23 et/ou DIA3 pour surexprimer ces gènes, et/ou par suppression d'un ou de plusieurs gènes régulateurs répresseurs PHO80 et/ou PHO85, l'insertion étant soit chromosomique soit effectuée par transformation de la souche au moyen d'un plasmide contenant le(s) gène(s).

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