Saf polypeptide, gene, promoter and use for expression in...

C - Chemistry – Metallurgy – 12 – N

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530/13, 195/1.2,

C12N 15/76 (2006.01) C07K 14/36 (2006.01) C12N 1/21 (2006.01) C12N 15/31 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2033069

The cloning and characterization of a newly isolated gene, referred to as saf (secondary metabolism activation factor) is disclosed. This gene encodes a new amino acid polypeptide, referred to as the SAF polypeptide, which directly or indirectly modulates the expression of extracellular enzymes in Streptomyces. DNA units or fragments which encode the SAF polypeptide are also disclosed as are vectors containing said DNA, host organisms transformed with such vectors, and processes for preparing extracellular enzymes or heterologous polypeptides by culturing such host organisms.

Le clonage et la caract'risation d'un gSne r'cemment isol' et d'sign' sous le nom de saf (facteur d'activation de m'tabolisme secondaire) sont divulgu's. Ledit gSne code un nouveau polypeptide d'amino-acide, d'sign' sous le nom de polypeptide SAF et modulant, directement ou indirectement, l'expression d'enzymes extracellulaires dans le Streptomyces. Des unit's ou des fragments d'ADN codant ledit polypeptide SAF sont 'galement divulgu's, ainsi que des vecteurs contenant ledit ADN, des organismes h"tes transform's par l'introduction desdits vecteurs, et les proc'd's pour la pr'paration d'enzymes extracellulaires ou de polypeptides par la culture de tels organismes h"tes.

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