Sample analysis with successive quanta timing code

G - Physics – 01 – N

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G01N 21/64 (2006.01) G01N 21/17 (2006.01) G01N 21/76 (2006.01)

Patent

CA 2349218

In analyzing radiation (15) from a sample (14), single-quanta counting can be used to advantage especially at low levels of radiation energy, e.g., in the detection of fluorescent radiation. Preferred detection techniques include methods in which (i) fluorescence-stimulating radiation (12) is intensity- modulated in accordance with a preselected code, (ii) fluorescent radiation (15) is intensity-modulated with the preselected code, and/or (iii) modulation with the preselected code is applied to the sample (14) to influence a property which functionally affects emitted fluorescent radiation (15). The time intervals between signals (18) from a sensing element of a single-photon detector (17) are determined, recorded, and compared with the preselected code.

Lorsqu'on analyse un rayonnement (15) provenant d'un échantillon (14), on peut utiliser le comptage des quanta uniques, afin de favoriser, en particulier, les faibles niveaux d'énergie de rayonnement, par exemple dans la détection d'un rayonnement fluorescent. Les techniques de détection préférées comprennent des procédés consistant (i) à moduler en intensité un rayonnement de fluorescence selon un code présélectionné, (ii) à moduler en intensité un rayonnement fluorescent (15) à l'aide du code présélectionné, et/ou (iii) et à appliquer cette modulation à un échantillon (14) à l'aide du code présélectionné, afin d'agir sur une propriété qui affecte fonctionnellement le rayonnement fluorescent (15) émis. On détermine, on enregistre, et on compare les intervalles de temps entre les signaux (18) provenant d'un élément de détection d'un détecteur de photons (17) à l'aide du code présélectionné.

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