Sample buffer and methods for high resolution gel...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) G01N 27/447 (2006.01) G01N 31/00 (2006.01)

Patent

CA 2318062

A sample buffer and method are provided for preparing denatured nucleic acids and applying them to gel electrophoresis sytems. The sample buffer and method enhance band flatness and sharpness, resulting in higher resolution and throughput.

L'invention concerne un tampon d'échantillon et un procédé correspondant, qui permettent d'élaborer des acides nucléiques dénaturés et d'appliquer ces acides nucléiques à des systèmes d'électrophorèse en gel. Le tampon d'échantillon et le procédé considérés améliorent la linéarité et la netteté de bande, moyennant quoi la résolution et le débit sont de meilleure qualité.

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