Sample mounts for microcrystal crystallography

G - Physics – 01 – N

Patent

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G01N 23/20 (2006.01)

Patent

CA 2519830

Sample mounts (10) for mounting microcrystals of biological macromolecules for X-ray crystallography are prepared by using patterned thin polyimide films (12) that have curvature imparted thereto, for example, by being attached to a curved outer surface of a small metal rod (16). The patterned film (12) preferably includes a tapered tip end (24) for holding a crystal. Preferably, a small sample aperture is disposed in the film for reception of the crystal. A second, larger aperture can also be provided that is connected to the sample aperture by a drainage channel, allowing removal of excess liquid and easier manipulation in viscous solutions. The curvature imparted to the film (12) increases the film's rigidity and allows a convenient scoop-like action for retrieving crystals. The polyimide contributes minimally to background and absorption, and can be treated to obtain desired hydrophobicity or hydrophilicity.

L'invention concerne des supports d'échantillons (10) destinés au montage de microcristaux de macromolécules biologiques pour une cristallographie par rayons X, préparés à partir de pellicules polyimide fines à motif (12) auxquelles ont a donné une courbure en les fixant, par exemple, à la surface externe courbe d'une petite tige de métal (16). La pellicule à motif (12) comprend de préférence une extrémité en pointe conique (24) pour maintenir le cristal. De préférence, la pellicule comprend une petite ouverture à échantillon permettant de recevoir le cristal. La pellicule peut également comprendre une seconde ouverture plus grande, reliée à l'ouverture à échantillon par un canal de drainage, permettant d'évacuer le liquide en excès et de manipuler plus facilement les solutions visqueuses. La courbure de la pellicule (12) augmente la rigidité de la pellicule et permet de ramasser facilement les cristaux. Le polyimide est très peu absorbant et peut être traité pour obtenir des caractéristiques hydrophobes ou hydrophiles désirées.

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