Sample processing device and method

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/36 (2006.01) C12M 1/34 (2006.01) C12M 1/38 (2006.01) C12Q 1/68 (2006.01) G01N 35/00 (2006.01) C12M 1/42 (2006.01) G01N 35/10 (2006.01)

Patent

CA 2433347

A device (10) for processing a biological sample includes a processing unit (12) having at least one opening (14) to receive a sample vessel (16) and a plurality of processing stations (18) positioned along the opening (14). The processing stations (18) each have a compression member (22) adapted to compress the sample vessel (16) within the opening (14) and thereby move the sample within the sample vessel (16) among the processing stations. An energy transfer element can be coupled to one or more of the processing stations for transferring thermal energy to the sample at a processing station. The device can be used for PCR processing of nucleic acid samples. A sample vessel (16) of the present invention can be a tubule flow-chamber having a plurality of segments separated by pressure gates. The sample vessel (16) minimizes sample handling by providing a closed tubule in which distinct processing steps can be carried out in each of the segments of the tubule.

L'invention concerne un dispositif (10) de traitement d'échantillons biologiques comportant une unité de traitement (12) présentant au moins une ouverture (14) pour recevoir un flacon à échantillon (16), et un groupe de plusieurs postes de traitement (18) placés le long de la dite ouverture (14). Ces postes de traitement (18) comprennent chacun un élément de compression (22) qui serre le flacon à échantillon (16) dans l'ouverture (14) et déplace ainsi l'échantillon contenu dans le flacon à échantillon (16) à travers les postes de traitement. On peut coupler un élément de transfert d'énergie à un ou plusieurs postes de traitement pour transférer l'énergie thermique à l'échantillon dans un poste de traitement. On peut utiliser ce dispositif pour un traitement PCR d'échantillons d'acides nucléiques. Un des flacons à échantillon (16) de l'invention peut être une chambre d'écoulement à tubules comportant une pluralité de segments séparés par des vannes à pression. Le flacon à échantillon (16) réduit au minimum la manipulation des échantillons grâce au tubule fermé dans lequel on peut réaliser des opérations de traitement distinctes dans chacun de ses segments.

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