Sample processing method for whole blood

Details Sample processing method for whole blood Sample processing method for whole blood

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) C12N 1/06 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2170967

Methods for processing whole blood for nucleic acid analysis of microorganisms which may be present are provided. The methods eliminate inhibitors which interfere in particular with enzymatic nucleic acid reactions and are also compatible with conventional culturing techniques. It has been found that selective lysis of red blood cells with certain lysis buffers, followed by centrifugation at 5,000-15,000 xg for 5-30 min. and washing with water, saline, physiological buffers, or buffers compatible with nucleic acid analysis removes inhibitors of nucleic acid reactions which are present in whole blood or are introduced by reagents used in the sample processing protocol. The method therefore allows nucleic acid analysis of large samples of whole blood without interference from inhibitors, thereby improving the sensitivity of the nucleic acid analysis.

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