C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) B01L 7/00 (2006.01) C12M 1/34 (2006.01) C12P 19/34 (2006.01) B01L 99/00 (2010.01)
Patent
CA 2509769
The invention provides sampling methods and apparatuses that permit the quantitative analysis of nucleic acid amplification reactions. Quantitative information garnered during an amplification regimen permits the development of a detailed amplification profile that in turn permits the reliable determination of original template abundance. The methods disclosed are particularly useful for quantitative expression analysis of multiple genes or transcription units, both in the same amplification reaction and in multiple amplification reactions.
L'invention concerne des procédés et dispositifs d'échantillonnage permettant d'analyser quantitativement des réactions d'amplification d'acide nucléique. Des informations quantitatives recueillies lors du déroulement d'un protocole d'amplification permettent de développer un profil d'amplification détaillé qui permet, à son tour, de déterminer de manière fiable la quantité de matrices initiales. Les procédés selon l'invention peuvent en particulier être utilisés pour analyser quantitativement l'expression de gènes ou unités de transcription multiples, non seulement lors de la même réaction d'amplification, mais aussi au cours de réactions d'amplification multiples.
Primera Biosystems
Torys Llp
LandOfFree
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