Sandwich immunoassay and monoclonal antibodies for comp,...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/16 (2006.01) C12N 5/071 (2010.01) C07K 16/18 (2006.01) C12N 5/10 (2006.01) C12P 21/08 (2006.01) G01N 33/543 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2396342

The present invention provides a new sensitive direct sandwich assay for determining the presence of COMP in a clinical sample. Two monoclonal antibodies directed against separate antigenic determinants of the COMP molecules are used in the assay. The invention also relates to three particularly advantageous monoclonal antibodies per se that are directed against human COMP. Cell cultures manufacturing these antibodies have been deposited according to the Budapest Treaty at Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, and have been assigned accesion numbers DSM ACC2406, DSM ACC2408 and DSM ACC2418, respectively. A diagnostic kit comprising at least two of these monoclonal antibodies also constitute a part of the present invention.

La présente invention concerne un nouveau dosage direct sensible en sandwich permettant de déterminer la présence de COMP dans un échantillon clinique. Dans ce dosage, on utilise deux anticorps monoclonaux dirigés contre des déterminants antigéniques séparés des molécules de COMP. L'invention concerne également trois anticorps monoclonaux per se particulièrement avantageux dirigés contre la protéine COMP humaine. Des cultures cellulaires destinées à la fabrication de ces anticorps ont été déposées conformément au Traité de Budapest auprès de la Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, respectivement sous les références DSM ACC2406, DSM ACC2408 et DSM ACC2418. La présente invention se rapporte en outre à une trousse de diagnostic comprenant au moins deux de ces anticorps monoclonaux.

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