Sandwich immunoassay kits for detecting ciguatoxin ctx3c

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/12 (2006.01) C07D 493/22 (2006.01) C07K 14/76 (2006.01) C07K 16/12 (2006.01) C07K 16/14 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2478658

A sandwich immunoassay kit for detecting ciguatoxins based on a combination of two anti-ciguatoxin CTX3C monoclonal antibodies produced by hybridomas, 3D11 (deposited at IPOD, AIST under accession number FMRM PB-8293) and 10C9 (FMRM PB-8292). In particular, one of the antibodies is labeled and each of them binds specifically to a different site of ciguatoxin CTX3C.

L'invention concerne des kits d'analyse <=en sandwich>= permettantde détecter des shigatoxines, qui comprennent une combinaison d'un anticorps monoclonal réagissant spécifiquement avec la shigatoxine CTX3C produite par un hybride 3D11 (numéro d'accession FERM BP-8293) et d'un autre anticorps monoclonal réagissant spécifiquement avec la shigatoxine CTX3C produite par un hybride 10C9 (numéro d'accession FERM BP-8293). L'invention concerne notamment une combinaison dans laquelle l'un des anticorps monoclonaux est lié à un composé de marquage afin de produire un anticorps monoclonal marqué, puis combiné avec un autre anticorps monoclonal non marqué.

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