Scalable bioreactor culture process and system for the...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/04 (2006.01) A01H 4/00 (2006.01) C12M 3/00 (2006.01)

Patent

CA 2372488

A bioreactor culture system and process for producing conifer somatic embryos comprise a closed vessel, a biomass immobilization matrix, a liquid culture medium recirculating equipment, and a gas control equipment. The biomass immobilization matrix is installed in a closed vessel, a liquid culture medium is introduced in the closed vessel, and the level of liquid culture medium in the closed vessel is kept lower than the biomass immobilization matrix. The liquid culture medium recirculating equipment sprays liquid culture medium from the closed vessel onto the biomass immobilization matrix to thereby irrigate the maturing immobilized biomass.

Selon cette invention, un système de culture en bioréacteur et un procédé de fabrication d'embryons somatiques de conifères comprennent une cuve close, une matrice d'immobilisation de biomasse, un équipement de recirculation de bouillon de culture liquide et un équipement de régulation des gaz. La matrice d'immobilisation de biomasse est installée dans une cuve close, un bouillon de culture liquide est introduit dans la cuve close et le niveau du bouillon de culture liquide dans la cuve close est maintenu plus bas que la matrice d'immobilisation de la biomasse. L'équipement de recirculation de bouillon de culture liquide pulvérise le bouillon de culture liquide depuis la cuve close sur la matrice d'immobilisation de la biomasse et arrose ainsi la biomasse en maturation immobilisée.

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