Scalable process for cultivating undifferentiated stem cells...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/071 (2010.01) C12N 5/0735 (2010.01) C12N 5/074 (2010.01) C12M 3/00 (2006.01)

Patent

CA 2595137

The invention relates to a process for cultivating undifferentiated stem cells in suspension and in particular to a method for cultivating stem cells on microcarriers in vessels. The method enables stem cells to be cultivated in a scalable process for the first time, without losing their pluripotentiality. Different types of vessels are suitable for cultivation, such as e.g. spinners and bioreactors. The option of precisely setting the cultivation conditions in said vessels aids the process of obtaining a pluripotentiality of the stem cells. The inventive method also permits large yields of stem cells of an unvarying quality to be produced.

L'invention concerne un processus pour la culture de cellules souches indifférenciées en suspension, et notamment un procédé pour la culture de cellules souches sur des microsupports dans des récipients. Le procédé selon l'invention permet, pour la première fois, de cultiver des cellules souches au cours d'un processus évolutif sans que ces cellules perdent leur pluripotence. Différents types de récipients conviennent à la culture, des réacteurs agités et des bioréacteurs par exemple. La possibilité de régler précisément les conditions de culture dans ces récipients est utile pour préserver la pluripotence des cellules souches. Le procédé selon l'invention permet également de produire de grandes quantités de cellules souches de qualité constante.

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