Scanning optical microscope

Details Scanning optical microscope Scanning optical microscope

G - Physics

02

B

G02B 21/00 (2006.01)

Patent

CA 2059126

2059126 9015352 PCTABS00002 The invention improves the resolution in a scanning confocal microscope by performing optical signal processing prior to detection. Such processing is achieved by an element (7, 39, 42) having either or both phase and amplitude weighting. The microscope may be a transmission, reflection, or fluorescent microscope. In a transmission microscope a laser (1) illuminates a small area of a sample (3). Light from this small area is focussed onto an optical processing element (7). Output from the element (7) is detected by a detector (11) via a pin hole (9) in a stop plate (10). Phase weighting of the element (7) may be formed by a rectangular grating (15), and amplitude weighting formed by a sinusoidal variation in absorption. One optical weighting function m(x, y) is cos (2.pi.Nx/.lambda.).cos(2.pi.Ny/.lambda.), where N is the numerical aperture of lenses (2, 6) and .lambda. is wavelength. An alternative optical weighting function m(x, y) is sign (cos(2.pi.Nx/.lambda.)).sign (cos(2.pi.Ny/.lambda.)).

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