Scattered total internal reflectance apparatus

Details Scattered total internal reflectance apparatus Scattered total internal reflectance apparatus

G - Physics

02

B

88/96

G02B 27/20 (2006.01) A61B 6/08 (2006.01)

Patent

CA 2022292

- 56 - SCATTERED TOTAL INTERNAL REFLECTANCE APPARATUS Abstract of the Disclosure A new immunoassay system is provided for the detection of ligands or ligand binding partners in solution in a heterogeneous format. The invention relies upon the detection of back scattered light from an evanescent wave disturbed by the presence of a colloidal gold label brought to the interface by an immunological reaction. The evanescent wave existing at the interface in turn is the result of a totally internally reflected incident light wave. Placement of the detector at a back angle above the critical angle insures a superior signal-to-noise ratio. Apparatus and methods for scanning, detecting and manipulating light including a scattered total internal reflectance immunoassay system are provided. ORD-82

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