Scattered total internal reflectance immunoassay system

Details Scattered total internal reflectance immunoassay system Scattered total internal reflectance immunoassay system

G - Physics

01

N

354/30, 167/44,

G01N 21/43 (2006.01) G01N 21/51 (2006.01) G01N 21/55 (2006.01) G01N 33/553 (2006.01) G01N 33/58 (2006.01) G01N 21/47 (2006.01)

Patent

CA 1317006

-58- SCATTERED TOTAL INTERNAL REFLECTANCE IMMUNOASSAY SYSTEM Abstract of the Disclosure A new immunoassay system is provided for the detection of ligands or ligand binding partners in solution in a heterogeneous format. The invention relies upon the detection of back scattered light from an evanescent wave disturbed by the presence of a colloidal gold label brought to the interface by an immunological reaction. The evanescent wave existing at the interface in turn is the result of a totally internally reflected incident light wave. Placement of the detector at a back angle above the critical angle insures a superior signal-to-noise ratio.

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