Schwann cells originating in myeloid interstitial cells

Details Schwann cells originating in myeloid interstitial cells Schwann cells originating in myeloid interstitial cells

C - Chemistry, Metallurgy

12

N

C12N 5/077 (2010.01) C12N 5/079 (2010.01) A61K 35/30 (2006.01) A61L 27/38 (2006.01) A61P 25/00 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2450789

There is provided a method of inducing bone marrow stromal cells to differentiate into bone marrow stromal cell-derived Schwann cells in vitro, comprising the steps of: collecting bone marrow stromal cells from bone marrow and culturing the cells in a standard essential culture medium supplemented with a serum; adding a reducing agent to the culture medium and further culturing the cells; adding a differentiation inducing agent to the culture medium and further culturing the cells; and adding a cyclic AMP-augmenting agent or a cyclic AMP analogue and/or a glial cell differentiation and survival stimulating factor to the culture medium, and further culturing the cells to obtain the bone marrow stromal cell-derived Schwann cells. There are also provided bone marrow stromal cell-derived Schwann cells obtained thereby and a pharmaceutical composition for neural regeneration that comprises them.

L'invention concerne un procédé pour différencier et induire in vitro les cellules myéloïdes interstitielles dans des cellules de Schwann provenant des cellules myéloïdes interstitielles, ledit procédé consistant en ce qui suit: recueillir les cellules myéloïdes interstitielles à partir de la moelle osseuse et les cultiver dans un milieu préparé par l'ajout de sérum à un milieu de base standard; ajouter un agent réducteur à la culture liquide puis poursuivre la culture; ajouter un agent de différenciation différent à la culture liquide et poursuivre la culture; et ajouter un agent augmentant l'AMP cyclique ou un analogue d'AMP cyclique et/ou un facteur fonctionnel de survie de gliocytes par différenciation à la culture liquide puis poursuivre la culture afin d'obtenir des cellules de Schwann provenant des cellules myéloïdes interstitielles. L'invention concerne aussi des compositions médicales destinées à la reconstruction de nerfs contenant les composés de l'invention.

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