C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/34 (2006.01) C12Q 1/18 (2006.01)
Patent
CA 2194983
The present invention is directed to a method for screening samples for the identification of agents exhibiting potential fungicidal and insecticidal activity for a wide variety of agricultural, medical and pharmaceutical uses. The method utilizes cells that comprise a plasmid-born CTS gene of Saccharomyces cerevisiae, which allows for over expression of chitinase. Compounds that inhibit a hydrolytic action on methyl- umbelliferyltriacetyl chitotriose, but which are not toxic to the cells are detected by decrease in conversion of substrate.
La présente invention concerne un procédé pour trier des échantillons en vue de l'identification d'agents présentant une activité fongicide et insecticide potentielle pour une grande variété d'usage agricoles, médicaux et pharmaceutiques. Ce procédé utilise des cellules qui comprennent un gène CTS de Saccharomyces cerevisiae, d'origine plasmidique, qui permet la surexpression de chitinase. Des composés qui inhibent une action hydrolytique sur le méthyl-umbelliferyltriacétyle chitotriose, sans être toxiques pour les cellules, sont détectés par une diminution du virage du substrat.
American Cyanamid Company
Basf Aktiengesellschaft
Smart & Biggar
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