Screening assay for antagonists of fgfr-mediated malignant...

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A01K 63/00 (2006.01) A61K 48/00 (2006.01) A61K 49/00 (2006.01) C07K 14/71 (2006.01) C12N 1/20 (2006.01) C12N 5/00 (2006.01) C12N 5/02 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01) C12P 21/04 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/567 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2360745

In vitro and in vivo screening assays for antagonists of fibroblast growth factor receptor (FGFR)-mediated malignant cell transformation are provided using stable cell lines genetically engineered to express a recombinant wild type or constitutively active mutant FGFR selected from FGFR1, FGFR2 and FGFR3, the malignant potential of said cell lines being modulated by said FGFR.

L'invention concerne des dosages par criblage, in vitro and in vivo, destinés à la recherche d'antagonistes de la transformation cellulaire induite par le récepteur du facteur de croissance du fibroblaste (FGFR), et dans lesquels on utilise des lignées cellulaires modifiées génétiquement de manière à exprimer un type sauvage recombiné ou un FGFR mutant, actif de manière constitutive, choisi parmi FGFR1, FGFR2 et FGFR3, le potentiel malin de ces lignées cellulaires étant modulé par ledit FGFR.

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