Screening assay for anti-hiv drugs

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/49 (2006.01) C07K 14/16 (2006.01) C12N 15/85 (2006.01) C12Q 1/02 (2006.01) C12Q 1/18 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/60 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2202048

The essential role of the Vpr protein of HIV in the progression of HIV infection is disclosed. Recognition of this role permits assay for potential drug candidates by virtue of their ability to interfere with the interaction of Vpr with its intracellular targets. Thus, cells that are modified to contain an expression system for the production of Vpr are cultured in the presence and absence of the drug candidate and the growth rates compared. The ability of a candidate to enhance the growth of a culture producing Vpr indicates its potential as an anti-AIDS therapeutic.

L'invention concerne le rôle essentiel de la protéine Vpr du VIH dans la progression de l'infection à VIH. La reconnaissance de ce rôle permet d'analyser certains médicaments candidats potentiels de par leur aptitude à s'interposer dans l'interaction de Vpr avec ses cibles intracellulaires. Ainsi, des cellules modifiées de sorte qu'elles contiennent un système d'expression pour la production de Vpr sont mises en culture en présence et en l'absence du médicament candidat et les vitesses de croissance sont comparées. La capacité d'un candidat d'augmenter la croissance d'une culture de Vpr est représentative de son potentiel en tant que moyen thérapeutique contre le SIDA.

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