C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12Q 1/02 (2006.01) C12Q 1/04 (2006.01) G01N 33/567 (2006.01) G01N 33/58 (2006.01) C12N 15/85 (2006.01) C12N 15/90 (2006.01)
Patent
CA 2423052
The present invention provides methods for screening for a molecule that antagonizes or agonizes RANK activity. One aspect of the invention involves the growth of RANK responsive cells in semi-solid medium, wherein exposure to a RANK antagonist promotes colony formation. Other aspects of the invention rely on promoter/reporter constructs using RANK responsive promoters derived from the MMP-9 and TRAP genes. Additional aspects of the invention exploit the ability of RANK to activate c-src activity, F-actin ring formation and CaPO4 resorption.
La présente invention concerne des méthodes destinées à cribler une molécule antagonisant ou agonisant l'activité de RANK. Dans un mode de réalisation, l'invention se rapporte à la croissance de cellules sensibles à RANK dans un milieu semi-solide, l'exposition à un antagoniste de RANK favorisant la formation de colonies. D'autres modes de réalisation de l'invention portent sur des constructions de promoteur/rapporteur utilisant les promoteurs sensibles à RANK issus des gènes MMP-9 et TRAP. En outre, l'invention utilise la capacité de RANK à stimuler l'activité de c-src, la formation cyclique de l'actine F et la résorption de CaPO¿4?.
Immunex Corporation
Smart & Biggar
LandOfFree
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