Screening compound libraries using an optical fiber array...

G - Physics – 01 – N

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G01N 21/00 (2006.01) C07K 16/00 (2006.01) C07K 16/30 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2496741

Disclosed is a method of screening Compounds wherein target cells are coated onto a population of microbeads, and wherein each microbead is coated with several cells of the same cellular type and has an assay and an assay reporter associated with it. Each of the cell-coated microbeads are positioned in a well formed in one end of a fiber which is part of an array of optical fibers, and the microbeads are contacted with the compounds. The results of the assay associated with a microbead are reported to the distal end of the fiber, and each fiber in the array so reports.

L'invention a trait à un procédé de criblage de composés, des cellules cibles étant déposées sur une population de microbilles, chaque microbille étant revêtue de plusieurs cellules de même type cellulaire, et un dosage et un rapporteur de dosage étant associés à chaque microbille. Chacune des microbilles revêtues de cellules sont placées dans un puits formé dans une extrémité d'une fibre faisant partie d'un réseau de fibres optiques, et les microbilles sont mises en contact avec les composés. Les résultats du dosage associé à une microbille sont transmis à l'extrémité distale de la fibre, et chaque fibre du réseau effectue une transmission similaire.

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