Screening for agents modulating tgf-beta cell signaling

G - Physics – 01 – N

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G01N 33/68 (2006.01) C12Q 1/25 (2006.01) G01N 33/50 (2006.01) G01N 33/566 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2383514

Methods are provided for identifying agents that modulate signaling mediated by transforming growth factor beta (TGF-.beta.) and members of the TGF-.beta. family, such as bone morphogenic protein (BMP). Such agents may be identified using screens that evaluate candidate agents for the ability to modulate Smad protein degradation. Agents identified as described herein may be used to augment or inhibit signaling mediated by one or more TGF-.beta. family members in a variety of cell types and for therapeutic purposes.

L'invention concerne des méthodes d'identification d'agents modulateurs de la signalisation dépendant du facteur de croissance transformant bêta (TGF-.beta.) et de membres de la famille TGF-.beta., tels que la protéine morphogénique osseuse (BMP). Ces agents peuvent être identifiés à l'aide de criblages permettant d'évaluer la capacité des agents candidats à moduler la dégradation de la protéine Smad. Les agents ainsi identifiés peuvent être utilisés pour augmenter ou inhiber la signalisation dépendant d'un ou de plusieurs membres de la famille TGF-.beta. dans divers types de cellules et à des fins thérapeutiques.

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