Screening for anti-cancer compounds using netrin-1 activity

G - Physics – 01 – N

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G01N 33/50 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2638974

The subject matter of the present invention relates to an in vitro method for the screening of anti-cancer compounds based on the capacity for these compound to interact with netrin-1 receptor and/or to inhibit the dimerization of the intracellular domain of the netrin-1 receptor expressed in tumor cells. The invention also relates to a method for predicting the presence of metastatic or aggressive cancer, or for determining the efficiency of an anti- cancer treatment based on the measuring of the expression level of netrin-1. The invention further comprises kits and compounds as a medicament for the treatment of cancer such as metastatic breast cancer, related to the overexpression of netrin-1 by the tumor cells.

La présente invention concerne un procédé de criblage in vitro de composés anticancéreux basé sur la capacité de ce composé à interagir avec le récepteur de la nétrine-1 et/ou à inhiber la dimérisation du domaine intracellulaire du récepteur de la nétrine-1 exprimée dans les cellules tumorales. L'invention concerne également un procédé permettant de prédire la présence d'un cancer métastatique ou agressif ou de déterminer l'efficacité d'un traitement anticancéreux sur la base du niveau d'expression de nétrine-1 mesuré. L'invention concerne en outre des kits et des composés utilisés comme médicaments pour traiter un cancer, tel que le cancer du sein métastatique, lié à la surexpression de nétrine-1 par les cellules tumorales.

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