Screening for blood coagulation defects using metal ions

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/56 (2006.01) G01N 33/86 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2334935

In vitro photometric method for qualitative screening and quantitative determination of the functional activity of components of the Protein C anticoagulant pathway of blood coagulation, comprising measuring the conversion rate of an exogenous substrate by an enzyme, the activity of which is related to the Protein C anticoagulant activity, in a blood sample of a human comprising coagulation factors and said exogenous substrate after at least partial activation of coagulation through the intrinsic, extrinsic or common pathway and triggering coagulation by adding calcium ions; and comparing said conversion rate with the conversion rate of a normal human blood sample determined in the same way, comprising adding further metal(s) ions to said sample; kits and reagents for use in said method.

Ce procédé photométrique in vitro de criblage qualitatif et de détermination quantitative de l'activité fonctionnelle de composants du mécanisme d'action anticoagulant de la protéine C de la coagulation sanguine consiste d'abord à mesurer le taux de conversion d'un substrat exogène par une enzyme dont l'activité est associée à l'activité anticoagulante de la protéine C, dans un échantillon sanguin humain comprenant des facteurs de coagulation et le substrat exogène, après au moins une activation partielle de la coagulation, par le biais du mécanisme d'action intrinsèque, extrinsèque ou commun, puis à déclencher la coagulation par ajout d'ions calcium, et enfin à comparer ledit taux de conversion avec le taux de conversion d'un échantillon sanguin humain normal, déterminé de la même manière, ce procédé étant caractérisé par un ajout d'autre(s) ion(s) métallique(s) audit échantillon. L'invention concerne également des trousses et réactifs utiles dans ce procédé.

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