Screening method

Details Screening method Screening method

G - Physics

01

N

G01N 33/50 (2006.01)

Patent

CA 2377684

The present invention provides a screening method for the identification of compounds which modulate an AMP-sensitive regulatory site on mitochondria comprising the steps of: a) contacting a test compound with mitochondria in the presence of a substrate for respiration in the presence of a buffer system; b) measuring an index of metabolic rate; and c) identifying compounds which modulate metabolic rate. Alternatively or additionally membrane potential can be measured. The invention also comprises a regulatory site for a mitochondrial proton leak which is related to the adenine nucleotide carrier, a binding assay and a functional assay.

La présente invention concerne un procédé de criblage utilisé pour identifier des composés qui modulent le site régulateur sensible à l'AMP des mitochondries consistant à: a) mettre en contact un composé d'essai avec les mitochondries en présence d'un substrat pour la respiration en présence d'un système tampon b) mesurer l'indice de la vitesse de métabolisme; et c) identifier les composés qui modulent la vitesse de métabolisme. Dans une variante/de plus, on peut mesurer le potentiel membranaire. En outre, cette invention concerne un site régulateur d'une fuite de protons des mitochondries lié au vecteur adénine-nucléotide, un dosage de liaison et un dosage fonctionnel.

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