Screening method for determining individuals at risk of...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2214461

The present invention relates to a screening method to identify individuals at risk of developing diseases associated with different polymorphic forms of wildtype p53; which comprises the steps of: a) obtaining a biological sample from said patients; and b) determining the presence of p53pro or p53arg wildtype alleles in said sample; wherein the allele pattern of patients selected from the group consisting of p53pro/p53pro, p53arg/p53arg and p53pro/p53arg are indicative of a risk factor for developing disease associated with different polymorphic forms of wildtype p53. Notably, individuals who are p53arg/arg are at greater risk of developing pathologies associated with human papillomavirus infections, including cervical cancer.

La présente invention porte sur une méthode de dépistage pour identifier les individus qui risquent de contracter des maladies associées à différentes formes polymorphiques du type sauvage p53. La méthode consiste à : a) obtenir un échantillon biologique des patients; b) à déterminer la présence d'allèles du type sauvage p53pro ou p53arg dans cet échantillon; le type d'allèle chez les patients choisis dans le groupe constitué de p53pro/p53pro, p53arg/p53arg et p53pro/p53arg est un indicateur du facteur de risque d'apparition de maladies associées avec différentes formes polymorphiques du type sauvage p53. Par exemple, les individus avec p53arg/arg courent un plus grand risque de contracter des pathologies associées aux infections par le virus des papillomes humains, y compris le cancer du col utérin.

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