C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/37 (2006.01)
Patent
CA 2598483
The invention relates to a method for identifying a protease secretion- deficient strain of a microorganism, during which a mutant of the ciliate is added to a gel and incubated under conditions in which the mutant of the ciliate secretes proteins, and the mutant of the ciliate is separated from the gel. At least one substrate for at least one secretion protease of the ciliate is contained in the gel and/or the gel, itself, is the substrate and/or the substrate is added later, and at least partially diffuses into the gel, and the protease activity on the substrate is measured.
L'invention concerne un procédé pour identifier une souche déficiente en sécrétion de protéase dans un micro-organisme, selon lequel un mutant du cilié est ajouté à un gel et mis en incubation dans des conditions où le mutant du cilié secrète des protéines, puis le mutant du cilié est séparé du gel, le gel contenant au moins un substrat pour au moins une protéase de sécrétion du cilié et/ou le gel étant lui-même le substrat et/ou le substrat étant ajouté ultérieurement et se diffusant au moins partiellement dans le gel, et l'activité de la protéase sur le substrat étant alors mesurée.
Broermann Andre
Hartmann Marcus
Cilian Ag
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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